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Home > chinese-english > "bacterial protein" in English

English translation for "bacterial protein"

细菌蛋白

Related Translations:
bacterial toxin:  细胞毒素细菌毒素
bacterial etiology:  细菌病因学
bacterial indicator:  细菌指示物
bacterial aggregae:  细菌团块
bacterial disease:  细菌病细菌性病害细菌性疾病
bacterial attack:  细菌腐蚀细菌侵袭
bacterial embolus:  细菌栓子
bacterial plaque:  齿斑块菌斑
bacterial spoilage:  细菌性腐败
bacterial antigen:  细菌性抗原
Example Sentences:
1.The amount of the goal protein was evaluated by densitometric scanning . it indicated that the product of the vp2 gene was 14 % of total bacterial protein of dh5a
表达产物经ni - nta金属鏊合层析柱纯化后,得到了纯度较高的6his - vp2融合蛋白。
2.The sds - page analysis revealed that the trka extracellular domains proteins were highly expressed and accumulated up to above 30 % of the total bacterial proteins after iptg induction
称a膜外域结构域重组蛋白表达量均占全菌蛋白的30 %以上。
3.The amount of the goal protein was evaluated by densitometric scanning . it indicated that the 45ku product of the vp6 gene was 26 . 5 % of total bacterial protein of bl21
表达产物经ni - nta金属螯合层析柱纯化后,得到纯化的his - taggedvp6融合蛋白。
4.Especially , the recombinant pet21a having 5 copy gene in e . coli bl21 ( de3 ) got high expression and expression level was up to 25 % of the total bacterial proteins
其中,含有5份串连基因的pet21a重组质粒在bl21 ( de3 )中的诱导表达量占总蛋白的25左右。
5.3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa . 2 after sds - page and densitometric scan analysis , the result show that expression level is 25 - 30 % of total bacterial proteins
山西医科大学2002届硕士学位论文一2dhsa pbv220 rhpf4经温控诱导表达后, sds page及凝胶密度扫描分析,表达产物占总国体蛋白的25 30 ,凝胶迁移特性与hpf4标准品相同。
6.After washing with reagent , adopt the newest purification technology source30rpc , sds - page and densitometric scan analysis , the result show that expression level is 90 % of total bacterial proteins . after renaturation , ifnr , hgfa , hgfb , hpk5 were purified by akta purifier chromatogram instrument , sepharose fast flow , ssphacrayl series gel , selecting optimize condition . finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies , purification product purity > 98 %
结论:总之,通过对发酵罐中重组工程菌各种培养因素的研究,建立了一种高密度、高表达发酵工艺体系,为重组蛋白的后续纯化提供了大量、稳定的原料供应;通过对不同目的蛋白的色谱行为的系统研究,建立了一种高效稳定、快速简洁、易于放大的包涵体重组蛋白分离纯化体系。
7.In this study , we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene . about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next , we construct recombinant plasmid pproex ? t - il - 2 . the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t - il - 2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il - 2 protein
经酶切鉴定及dna序列测定,该基因为鸡il - 2基因,其序列与sundick等报道的完全一致。在此基础上,我们把鸡il - 2基因亚克隆到大肠杆菌原核表达载体pproex ~ ( tm ) ht中,构建重组表达质粒并进行确证性序列测定,重组质粒测序结果表明将编码鸡il - 2成熟蛋白的基因正确地插入到原核表达载体pproex ~ ( tm ) ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养, sds - page和westernblot分析显示,表达的鸡il - 2融合蛋白分子量约为18kda ,表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30 。
8.Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins , and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda , which consisting 35 % of total bacterial proteins . 5
Sds - page分析表明,重组质粒pet - 32a ( + ) - igf -在iptg诱导下表达分子量约30kda的融合蛋白,但其表达量不高,约为菌体总蛋白的10左右;重组质粒pet - 30a ( + ) - igf -在iptg诱导下表达分子量约14kda的重组蛋白,融合蛋白表达量约占菌体蛋白总量的35 。
9.The recombinant expression plasmid was transformed into bl21 ( de3 ) and gene expression was induced by administration of iptg . expression was detected by sds - page and confirmed by western blot analysis , which shows that the yield of the target protein was approximately 30 % of the total bacterial protein
将这个表达载体转化入大肠杆菌表达菌株bl21 ( de3 )中, iptg诱导培养后,经sds - page和western杂交实验检测可知特异性表达带约在44kd的位置,其表达量大约占细菌总蛋白的30左右。
10.Ptxb1 - hng and ptxb1 - m - insulin are expressed in e . coli successfully . after sds - page and densitometric scan analysis , the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins . western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )
Pbv220 ? hng在大肠杆菌中未检测到表达,后两个克隆在大肠杆菌bl21 ( de3 )中获得高效表达, hng及m - insulin融合蛋白表达量分别占全菌蛋白的40及50左右;经western - blot鉴定m - insulin融合蛋白可以与小鼠抗人胰岛素单克隆抗体( igg )发生抗原抗体结合反应。
Similar Words:
"bacterial pro statitis" English translation, "bacterial product" English translation, "bacterial proof filter" English translation, "bacterial prospecting" English translation, "bacterial prostatitis" English translation, "bacterial proteinase" English translation, "bacterial proteines" English translation, "bacterial proteolysis" English translation, "bacterial pustule" English translation, "bacterial pustule of soybean" English translation